Abstract

ABSTRACT

Mansonia gagei J.R.Drumm. has long been utilized in traditional medicine and is recognized as a rich source of bioactive natural compounds. Among them, mansonone G and mansonone E are two major constituents strongly associated with diverse pharmacological activities. However, no analytical study to date has reported a validated method for their simultaneous quantification to ensure extract quality. This study aimed to survey the chromatographic conditions and validate the HPLC method in accordance with ICH and AOAC guidelines. The results demonstrated that the Poroshell 120 EC-C18 column (3.0 × 100 mm; 2.7 μm) provided superior separation compared to the Cosmosil 5C18-ARII column (250 × 4.6 mm; 5 μm), achieving retention time of 4.8 min (mansonone E) and 5.2 min (mansonone G) with a resolution of 3.7. The method was validated for specificity, linearity, accuracy, precision, and system suitability. This validated analytical procedure offers a reliable approach for quantifying Mansonia gagei extracts and serves as a foundation for establishing quality standards for herbal formulations derived from this promising species.